This course provides theoretical and practical background useful for engaging in cutting-edge chemical separations in chemistry, biology, medicine, engineering, research, and industry. The course covers general separations concepts and principles, with an emphasis on liquid chromatography and its various modes, including partition chromatography, ion chromatography, enantiomer chromatography, size exclusion chromatography, and affinity chromatography. Other topics include materials and instrumentation, gas chromatography, supercritical fluid chromatography, electrophoresis and related techniques, and a host of miscellaneous separation (e.g., TLC, FFF, CF) and extraction (e.g., LLE, SPE, SPME) modalities. Classes are supplemented with online/virtual laboratory exercises.