This course provides in depth foundational knowledge underlying molecular biology technologies. It focuses on how genetic principles were applied to develop the most commonly used molecular biology techniques today. Students will expand their genetic knowledge from introductory level genetic courses. This course does not involve explaining the basics of molecular biology such as replication, recombination, translation or gene expression. By contrast, this course expects that students already understand these concepts in detail and now apply this understanding towards how they were used to develop experimental technologies. For example, how are transformation, mutagenesis, plasmid construction, and gene expression systems used to design molecular experiments.